DNA polymerase preference determines PCR priming efficiency
نویسندگان
چکیده
منابع مشابه
DNA polymerase preference determines PCR priming efficiency
BACKGROUND Polymerase chain reaction (PCR) is one of the most important developments in modern biotechnology. However, PCR is known to introduce biases, especially during multiplex reactions. Recent studies have implicated the DNA polymerase as the primary source of bias, particularly initiation of polymerization on the template strand. In our study, amplification from a synthetic library conta...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2014
ISSN: 1472-6750
DOI: 10.1186/1472-6750-14-10